Chlamydia protocols

• Resuscitating McCoy cells
• Passaging McCoy cells
• Infecting cells with chlamydia
• Harvesting chlamydia
• Immunofluorescence staining
• X-gal titration

General information

Media

We use Gibco DMEM medium (Glutamax, -Pyruvate) from Life Technologies; we supplement this with foetal calf serum (FCS) to a final concentration of 10%.

Cell lines

We only use verified cell lines supplied by the NCTC. In most of our work we use mouse McCoy cells, but chlamydia will grow in many different cell types (e.g. HEp2, BGM, HeLa) and we recommend that you review current literature as to whether you can use other cell lines.

Please Note: the information in these pages are for guidance only and we cannot guarantee the success of your experiments. If you encounter problems, please contact us and we will try to help.